4/10/2023 0 Comments Canary mail ativity stream![]() ![]() Gas Chromatography/Mass Spectrometry AnalysisĮssential oil constituents were analyzed by a GC/MS QP-5000® (Shimadzu, Japan) gas chromatograph equipped with an electron ionization mass spectrometer. Oil was stored at ≤º C until tested and analyzed. The sample was submitted for six hours to steam distillation using a Clevenger apparatus to produce 0.9 mL of a rich essential oil extract from 1.373 g of sample, yielding 0.06% (v/w) (22). Propolis samples were collected by Apis mellifera bees in Rio de Janeiro, during Brazilian autumn time (July) and was supplied by a beekeeper co-operative (COAPI-RJ). In this study, we report the chemical composition of propolis essential oil and its capacity to inhibit the in vitro growth of Staphylococcus aureus, Staphylococcus epidermides, Streptcoccus pyogenes and Escherichia coli. ![]() Though the antibacterial activity of propolis essential oil from Paraná and Piauí states has been reported, the literature on the antimicrobial activity of this oil from Rio de Janeiro State is scarce (17, 21). The chemical composition of the analyzed essential oils showed qualitative and quantitative variations due to the influence of local soil conditions and seasonal harvest periods (15). Many essential oils from propolis have been analyzed and chemical substances such as terpenoids, alcohols, aldehydes, hydrocarbons and aliphatic ketones have been reported (14-20). (13) observed in vitro activity of propolis extracts and supported the efficaciousness of this natural drug against Streptcoccus pyogenes, a microorganism responsible for several otorhinolaryngological infections. In addition, propolis is known to be a health food that presents antibacterial, antioxidative, immunomodulatory, antifungal, antiviral, antiprotozoal, anti-inflammatory and antitumor activities (3-12). Resin is masticated, salivary enzymes are added and the partially digested material is mixed with beeswax and used in the hive (1, 2). Honeybees ( Apis mellifera L.) collect the resin from tree bark and leaf buds. Propolis is a resinous substance collected, transformed and used by bees to seal holes in their honeycombs, smooth out the internal walls and protect the hive entrance against intruders. Key words: Apis mellifera, propolis essential oil, terpenoids, antibacterial activity. IIILaboratory of Natural Product Technology, School of Pharmacy, Fluminense Federal University, Niterói, Rio de Janeiro State, Brazil IILaboratory of Microbiological Control, School of Pharmacy, Fluminense Federal University, Niterói, Rio de Janeiro State, Brazil IResearch Group of Natural Products, Health Sciences Center, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro State, Brazil Oliveira AP I França HS I Kuster RM I Teixeira LA II Rocha LM III This oil also exhibited antibacterial activity.Īpis mellifera propolis essential oil terpenoids antibacterial activityĬhemical composition and antibacterial activity of Brazilian propolis essential oil New components, namely linalool, methyl hydrocinnamate, ethyl hydrocinnamate, α-ylangene, γ-elemene and valencene, are reported for the first time to be present in propolis essential oil. From the 26 identified constituents, β-caryophyllene (12.7%), acetophenone (12.3%) and β-farnesene (9.2%) were found to be major components. Antibacterial activity was measured as equivalent diameters of inhibition zones (in millimeters) after incubation at 37º C for 24 hours. In addition, the agar diffusion method using filter paper disks was employed. The essential oil was obtained by steam distillation of propolis and examined by gas chromatography/mass spectrometry (GC/MS). The present study aimed at investigating the chemical composition of essential oil extracted from Brazilian propolis and the susceptibility of Staphylococcus aureus, Staphylococcus epidermides, Streptococcus pyogenes and Escherichia coli to this substance. ![]()
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